Journal: Antibodies

Article Title: Cryopreservation of Natural Killer Cells Pre-Complexed with Innate Cell Engagers

doi: 10.3390/antib11010012

Figure Lengend Snippet: Specificity of bispecific ICE ® constructs to induce EpCAM + tumor cell lysis. Calcein-labeled KARPAS-299 ( A ), HCC-1954 ( B ), Detroit 562 ( C ), and HCC-1187 cells ( D ) were co-cultured with enriched primary human NK cells as effector cells at an E:T ratio of 5:1 in the presence of serial dilutions of the indicated antibodies. After a 4 h incubation, the levels of calcein fluorescence released from the lysed target cells into the supernatant were quantified and used for the calculation of the percentage of specific lysis. Mean of duplicate values ± standard deviation are plotted. For specific constructs in different cell lines, fitting of sigmoidal dose–response curves was not possible. E:T, effector-cell-to-target-cell ratio.

Article Snippet: The KARPAS-299 cell line (Cat. no. ACC 31) was purchased from DSMZ (Braunschweig, Germany).

Techniques: Construct, Lysis, Labeling, Cell Culture, Incubation, Fluorescence, Standard Deviation

Journal: Antibodies

Article Title: Cryopreservation of Natural Killer Cells Pre-Complexed with Innate Cell Engagers

doi: 10.3390/antib11010012

Figure Lengend Snippet: Cytotoxicity of cryopreserved NK cells pre-complexed with ICE ® constructs against HCC-1187 and KARPAS-299 target cells. NK cells as effector cells were pre-complexed with 10 µg/mL of the indicated ICE ®® constructs and antibodies and frozen at −80 °C. As control samples, aliquots of the same NK cells were pre-incubated without an antibody (w/o antibody) and subjected to one freeze/thaw cycle. Thawed NK cells were washed and directly used as effector cells at the indicated E:T ratios. Where indicated, ICE ® constructs (EpCAM/NKp46, EpCAM/NKG2D, EpCAM/CD16A, or CD30/CD16A) were freshly added to NK cells that were not pre-complexed at a concentration of 10 µg/mL to the cytotoxicity assays against HCC-1187 ( A ) and KARPAS-299 ( B ) target cells. The mean ± standard deviation of duplicate lysis values were plotted. E:T, effector-cell-to-target-cell ratio; w/o, without.

Article Snippet: The KARPAS-299 cell line (Cat. no. ACC 31) was purchased from DSMZ (Braunschweig, Germany).

Techniques: Construct, Incubation, Concentration Assay, Standard Deviation, Lysis